The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. The MTT assay was performed with 100 μM vitamin E in the presence or absence of LPS at a concentration of 1 µg/mL for 24 h. 3. 8 Pages. 9. Fluorescence was measured with excitation and emission wavelengths of 530 and 590 nm, respectively, on a FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Corp. 6. Z’-LYTE® Compatible Microplate Reader Documentation Version No. A microplate reader (also called sometimes microtiter plate reader) is a high-sensitivity instrument designed to analyze samples contained in microplate wells. 2 Pages. FilterMax F3 / FilterMax F5: Inquire: SpectraMax iD3 and iD5: SpectraMax M: Price: Description: Unlimited breadth of user-upgradeable application modules expands research capabilities. Neurotransmitter Transporter Uptake Assay Kit. SpectraMax i3x Multi-Mode Detection Platform Biotek Synergy H1 Operator's Manual Appliskan User Manual FilterMax F3/F5 Multi- Mode Microplate Readers Spectramax® m2/m2 user guide User manual Software version 3. MD, MPH, Milton J. used. 01% in 2030. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. Eight replicates of three independent biological samples for each experimental condition (n = 24) were used to validate the collected data. ScanLater Western Blot Detection System. For 3D cell proliferation assay, (high concentrations) in 3D cellular proliferation assay, 20, 100 or 500 µg/ ml MG were used. The values of luminescence are directly proportional to the number of viable cells. Instrument Configuration: Assay Technology Adapta® & Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET only) (TR-FRET only) HTRF Certification LanthaScreen Certification Key. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumPage 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. A FilterMax F5 multi-mode microplate reader (Molecular Devices, San Jose, USA) was used to determine the absorbance (OD 450nm -OD 570nm). SpectraMax iD5. F5 Multi-Mode Microplate Reader. Perception (Vial Rack Bottom Label 2D Reader) Microscan. Neurotransmitter Transporter Uptake Assay Kit. com Check our website for a current listing SpectraMax i3/i3x Multi-Mode Microplate Reader SpectraMax Paradigm Multi-Mode Microplate Reader MOLECULAR DEVICES The trademarks used herein are the. Synergy HT laboratory equipment pdf manual download. Perkin Elmer Wallac Victor2 1420 Multilabel. 7/4/2018 Great Multi-Mode Microplate Reader Chirag Patel Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. Molecular Devices Multi-Mode Microplate Reader FilterMax F5 $7,995. GenePix 4300/4400 Microarray Scanner. 100 fmol 1536-well (8 µL) 0. 00 USD. The measurement can be performed using a variety of detection technologies: the. BioStack 4 offers patented plate de-lidding and re-lidding for sensitive cell-based workflows. Plates were read at absorbance 405 nm using a FilterMax F5 multi-mode microplate reader (Molecular Devices, Sunnyvale, CA). Multi-mode microplate readers are used in different research fields for the quantification of different biological and chemical assays in a microplate. 5% (v/v) to avoid toxicity. 7/4/2018 Great Multi-Mode Microplate Reader Chirag Patel Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. Jurkat cells were plated in a 12-well dish at a density of 8 x 10 5 cells in 1 mL media. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . 020 fmol 0. Technical specifications Compatible with these Molecular Devices systems Contact Us Phone: +1-800-635-5577 Email: [email protected]. These assays span over a wide wavelength range, generally from 220-900 nm in different detection modes (typically absorbance, fluorescence and luminescence). The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm), Emission Filter Slide #1 (Fluorescence: 535. The FilterMax F3 features absorbance (visible), fluorscence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. BioStack Neo is a dedicated stacker offering super-FilterMax™ F3 and FilterMax™ F5 Multi-Mode Microplate Reader User Guide 5008528 A September 2010 DTX Multimode Readers User Guide. Neurotransmitter Transporter Uptake Assay Kit. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader. Please note it is NOT a Keyspan USA-19 HS USB adaptor that is commonly used by. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. After 30 min, the reaction was stopped by adding 1 N HCl and absorbance at a wavelength of 490 nm was measured using the FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Corp. This assay measures cellular metabolic activity as an indicator of cell viability and proliferation. Load prepared PicoGreen plate (step C. FilterMax F5 Multi-Mode Microplate Reader. Add to cart. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader $ 7,900. Location: San Diego, California, UNITED STATES Member Since: 17th September, 2003 (20 years ago) Current Listings: 667 Shipping Policy: Freight Shipping and Handling: For all orders, Name/Company Name, Address, and Contact Phone Number are required. The absorption was measured in a spectrophotometer ultraviolet–visible (UV–Vis) Filter Max F5 multi-mode microplate reader; a wavelength of 595 nm was taken as the standard value for bacteria. Chemiluminescence emission was measured in real time (every 2 min for 120 min) with a FilterMax F5 Multi-Mode Microplate Reader at 27°C and expressed as area under the curve, as previously described . 0´ 105 dsD NA (p g/ wel ) R F U • Gemini™ EM/XPS Fluorescence Microplate Readers A B C Optimal data qualityGeneral Laboratory Instruments. All Synergy HT models support the reading of 6-, 12-, 24-, 48-, 96-, and 384-well microplates with standard 128 x 86 mm geometry. KG (CAT#: STEM-LE-1659-Y)The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seAfter 2 hours, each well was transferred to a 96-well flat-bottom plate and the absorbance at 490 nm was measured on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA). Biofilm quantification and scanning electron microscopy (SEM) analysis. Fluorescence was measured using a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA, USA). 1 at 600 nm as determined by FilterMax F5 multi-mode microplate readers (Molecular Devices). ] Example of document to evaluate if the reader . Maximum haemolysis was obtained using ultrapure distilled water, and untreated erythrocytes were used as a negative control [14]. Renilla luciferase activity was normalized to the background Firefly luciferase activity produced from the same psiCHECK-2 vector for each condition. The experiment was carried out with two biological replicates and three technical replicates. ABS Test results. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader w/ One Year Warranty (Svc Contract Available)Find used microplate readers on Machinio to perform a wide range of assays including ELISAs, cell viability assays, nucleic acid quantification, and more. --> Life sciences. In . Transfluor Technology. Samples were considered positive if the average sample absorbance was greater than twice the mean absorbance of healthy. Microplate readers read biological, chemical or physical events that occur on a microtiter plate. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Luminescence (glow) Fluorescence polarization. Create an experiment based on the protocol. 2 mm) was measured by a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices) every 15 min over 16 h incubation time. SpectraMax QuickDrop UV-Vis Spectrophotometer. SpectraMax iD5. Gently pull the plastic tab protruding from the microplate chamber door. CBUF CBUF CBUF CBUF CBUF CBUF CBUF CBUF CBUF CBUF. The FilterMax™ F5 features absorbance (UV and visible), fluorescence intensity - FI (top and bottom reading), glow luminescence, fluorescence polarization (FP), and time-resolved fluorescence (TRF) for 6- to 1536. * Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Molecular Devices for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above. Turn on the power to the FilterMax F5. . Upgradeable modules are also available including western blot, cell imaging, and fast. Close Search. FilterMax F3 and F5 Multi-Mode Microplate Readers; Absorbance Readers. 100 fmol 1536-well (8 µL) 0. All ELISA kits were read using a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices; San Jose, CA, USA), according to the kit guidelines. , Shanghai, China) and microplate reader (FilterMax F5 Multi-Mode Microplate Reader, LLC, Molecular Devices, San Jose, CA, United States) at a wavelength of 450 nm. SpectraMax MiniMax 300 Imaging Cytometer for the SpectraMax i3x Multi-Mode Microplate Detection Platform KEY FEATURES • ombine cellular imaging C and microplate assays into a single workflow • liminate cell staining for cell E counting and confluency with StainFree Technology • omplete cell viability C and toxicity assays with. Cell growth and viability were assessed using the CCK-8 proliferation assay. SpectraMax Glo Steady-Luc Reporter Assay Kit. 012 fmol 0. MDA is one of the final products of LPO. BMG Labtech - FLUOSTAR OMEGA. Instrument Configuration: Assay Technology Adapta® & After 30 min, the absorbance of the lysates was measured at 405 nm using a FilterMax F5 Multi-Mode microplate reader. Cytokines Assay. 2 Pages. Configurability is an important aspect of BioTek’s multi-mode readers to provide the most value for laboratory budgets, but the independent optical systems in our multi-mode readers don’t compromise on performance. The standard curve presented a linear phase from 1. Page 1 Hybrid Multi-Mode Microplate Reader Synergy ™ Operator’s Manual. Compare Fluorescence Microplate Readers from leading suppliers on Biocompare. F3/F5 Multi-Mode Microplate Readers. 4. The experiment was performed in 4 technical replicates (wells in the cell culture plate). The performance characteristics of the data reduction software have not been established with any laboratory diagnostic assay. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. The absorbance of samples at 405 nm (A 405) was determined at room temperature at 20 and/or 60 min after addition of substrate using a FilterMax F5 Multi-Mode Microplate reader (Molecular Devices, San Jose, CA). The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. 97059-524EA 84727. & FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. Molecular Devices 1311 Orleans Drive Sunnyvale, California 94089 Telephone: +1-408. After the absorbance was measured at 450 nm on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA), the concentrations of the samples were determined by. Sunnyvale, Calif. SpectraMax Mini Multi-Mode Microplate Reader. Multi-Mode Microplate Reader. In addition, the FilterMax F5 Multi-Mode Microplate Do you want to learn how to use the FilterMax F3 and F5 Multi-Mode Microplate Readers for your research? Download the user guide and find out how to install, operate, and troubleshoot the instruments. Optical System: High energy xenon flash lamp. Both the FilterMax F3 and F5 Readers are capable of detecting nucleic acid concentration by fluorogenic means. State-of-the-art Multi-Mode Microplate Reader. After initial incubation, the lysates were incubated with the caspase-1 substrate Ac-YVAD-pNA at 37 °C. Download Table of Contents. ] Example of document to evaluate if the reader . Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. For FcγRI (high affinity receptor) binding assays, plates were coated with 1 μg/mL recombinant human soluble. The ELISA test was performed using the supernatants from the morphological test. F3 F5 F3-SC F5-SC F3 F5. Assay buffer and 100% DMSO were used as vehicle controls. The transmittance at 450 nm was determined by FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA). If using a multi-functional reader, please be sure to check the sensitivity of the device using the MycoAlert™ Assay Control Set. Excitation Filter – EXP1 F535/25, F485/20, F485/20, F370/TRF. Add to my manuals. RLUs were determined using a FilterMax F5 multi-mode microplate reader (Beckman Coulter). Fluorescent intensity was measured at excitation (485 nm) and emission (595 nm) with the FilterMax F5 Multi-mode microplate reader (Molecular Devices). Background light emission was measured using the luminescence setting on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Techniques). GPCRs. A standard curve was created by diluting the ATP standards to a range between 2 × 10 −3 and 20 μM ATP. We have 2 Molecular Devices FilterMax F5 manuals available for free PDF download:. Once the FilterMax F5 has completely powered on, double-click on the Multi- Mode Analysis Software. The readings of samples incubated with different carbon sources were subtracted from the average of the control readings. Absorbance detection in the UV and visible range (230 nm to 650 nm) Fluorescence intensity; top read and bottom read. 4 fmol/100 μl fluorescein in Fluorescence Intensity-Top Read Mode, 0. Ammonia and NO 2 Ϫ / NO 3 Ϫ analyses were conducted according to established protocols (37, 38), using a FilterMax F5 multimode microplate reader (Molecular Devices, Canada). Supernatant (25 μL), or standard, was added and mixed. 8 Pages. MetaFluor Software. Cells were incubated at room temperature in the dark for 10 min, then luminescence was detected and quantified with the FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Sunnyvale, CA, USA). Page 154: Ordering Parts And Accessories. F5 Multi-Mode Microplate Reader. 00. Laser-aided direct metal deposition of metals and alloys. Download Table of Contents. 2 μM ATP standard was. Labsystems Fluoroskan Ascent FL Microplate Fluorometer $ 3,250. Upgradeable modules are also available including western blot, cell imaging, and fast. This instrument has passed specific verification criteria. microplate reader. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). The quantity of colorimetric product was continuously monitored for 120 min. FLIPR Membrane Potential Assay Kits. CloneMatrix. Transfluor Technology. Instrument Configuration: Assay Technology Adapta® & Setup for GeneBLAzer® Assay on Molecular Devices FilterMax™ F5 Microplate Reader with SoftMax® Pro 6. Products : General, FilterMax F5 reader Please click on the links below to view the Microplate Reader Comparison Tables. Caspase-3/7 activity, an indicator of apoptosis, was measured by a luminescence assay using caspase-Glo. Category 2 and 3 microplate readers use gradient heating as well. Mettler Jr. Our SpectraTest® ABS2, FL1, LM1, and Multi-Mode Validation Packages provide automated,A delightfully easy to use multimode reader. 6% from 2020 to 2027. FilterMax F3 & F5 Multi-Mode Microplate Readers. Click “Eject Plate Carrier. Citations: Dual monochromators increase performance and assay flexibility. Supplier: Molecular Devices. The solution was incubated for 15 min and fluorescence was measured on a SpectraMax microplate reader (Molecular Devices). x. Page 4 Operator’s Manual Intended Use Statement • The Synergy H1 is a hybrid multi-mode microplate reader. ScanLater Western Blot Detection System. The. After further centrifugation in the same fashion, the supernatant was subjected to subsequent enzymatic reactions. SpectraMax Glo Steady-Luc Reporter Assay Kit. Absorbance Detection Comparison Table. Plaque reduction. The increase in NADH, an end product in this reaction, was determined by means of its light absorbance at 355 nm using a fluorometric microplate reader (FilterMax F5 Multi-Mode Microplate Reader; Molecular Devices,. 7°C (gradient heating design). FilterMax™ F3 and FilterMax™ F5 Multi-Mode Microplate Reader User Guide 5008528 A September 2010 DTX Multimode Readers User Guide. A Vision for the Future With TTP LabTech. 375% (w/v) thiobarbituric acid was added. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. 1 at 600 nm as determined using a FilterMax F5 multi-mode microplate reader (Molecular Devices). FilterMax F3 & F5 Microplate Readers. 2. 2 Pages. HEK293T cells were harvested 48 h post-transfection in Passive Lysis Buffer (Promega). LDH cytotoxicity assaySome of new FilterMax F5 Multi-Mode Microplate readers are shipped with ARP USB to serial port adaptor (Manufacturer No: 572213, ARP No: 465338, 1 X USB, 1 X serial DB9/m, blue). Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. MetaFluor Software. Plate chamber design of the SpectraMax category 1 microplate readers. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. 005 OD. Click to Contact Seller. Fluorescence polarization was measured at 21°C using FilterMax™ F5 Multimode Microplate Reader (Molecular Devices) with excitation and emission wavelengths of 485 nm and 535 nm, respectively. Protease activities were calculated from the slope of the linear portion of the normalized fluorescence vs. Zebrafish whole-eye mRNA extraction and RT-PCRSearch LabX. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument se Figure 1: Schematic of a typical workow of the semi solid cloning process. There is no programming of protocols or wet chemistry involved –just select the mode(s) you want to test, insert the plate and go. Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 00. URL of this page: HTML Link: Bookmark this page. Homogeneous Time-Resolved Fluorescence (HTRF) . F3/F5 Multi-Mode Microplate ReadersThe plate was incubated for 1 hr at room temperature with shaking. HERM LB 500 with NaI detector Radio HPLC Detector. Renilla luciferase activity was normalized to the background Firefly luciferase activity produced from the same psiCHECK-2 vector for each condition. Supplier: Molecular Devices. Installation Qualification (IQ) FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and. Renilla/Firefly luciferase activities were measured twice. HTRF-compatible SpectraMax Multi-Mode Readers. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. Consult Molecular Devices's entire F3/F5 Multi-Mode Microplate Readers catalogue on MedicalExpo. Fluorescence of the organic phase was measured at 515 nm of excitation and 553-nm emission using a fluorimeter (FilterMax F5-Multi-Mode Microplate Reader, Molecular Devices, CA, USA). 5. Description Z-height focusing is enabled for fast top- and bottom-reads for all read modes. Cells were incubated at room temperature in the dark for 10 min, then luminescence was detected and quantified with the FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Sunnyvale, CA, USA). RLUs were determined using a FilterMax F5 multi-mode microplate reader (Beckman Coulter). FAST AND ACCURATE METERTECH Microplate Reader $3,650. Internal controls were. Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader + + Molecular Devices FlexStation. View specifications, prices, citations, reviews, and more. LVF Monochromator™ : Excitation and Emission. 00 SpectraMax M3, M4, M5, and M5e Multi-Mode Document type: User Manual5016208D 8 Chapter1:Introduction TheFilterMax™ F3Multi-ModeMicroplateReaderandtheFilterMax™F5Multi-Mode MicroplateReaderareidealforabroadrangeofapplications. com A. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile. Related Instruments Agilent BioTek BioStack microplate stacker Automate routine microplate washing or dispensing processes with the compact BioStack microplate stacker. [The Multi-Mode Validation Plate offers additional testing of TRF, HTRF, and fluorescence polarization read modes on FilterMax™ F3/F5 and SpectraMax® i3x, iD5, and Paradigm® microplate readers. 400. Turn on the power switch on the back of the instrument. Consult Molecular Devices's entire F3/F5 Multi-Mode Microplate Readers catalogue on MedicalExpo. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . shopping cart 0 items $0. 0. 1% (v/v) formic acid, was adopted for lipid separation: 0–2 min. HEK-Blue-hTLR4 and HEK-Blue-hTLR2 cells were incubated with. At least three independent experiments were performed for each. i3x, SpectraMax M4 or M5, and FilterMax F5 Multi-Mode Microplate Readers. All configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking and comprehensive data management using our SoftMax® Pro Microplate Data Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. 3 Pages. Align the two slots on the bottle holder with the two pegs on the rail. FilterMax F5 Multi-Mode Microplate Reader. Caspase-3 activity was used as a marker of cell apoptosis and was assessed according to. The FilterMax F5 features absorbance (UV and visible), fluorescence intensity - FI (top and bottom reading), glow luminescence, fluorescence polarization (FP), time-resolved fluorescence (TRF), for 6- to 384-well plates. Then 100 μL of 2 mol L −1 sulphuric acid was added to stop the enzymatic reaction and the absorbance values were read at 450 nm on a FilterMax F5 Multi-Mode Microplate Reader. There is no programming of protocols or wet chemistry involved –just select the mode(s) you want to test, insert the plate and go. J. They provide absorbance, fluorescence, glow luminescence, and other read modes for. This can increase throughput and efficiency, thus saving time and space in the lab. Recommended Optics P/N Filter information 6590-0048 FilterMax™ Fluorescence Excitation Filter 405 nm (bandwidth 30 nm) FilterMax F3 Multi-Mode Microplate Reader Includes Excitation Filter Slide #3 (Absorbance: 405 nm, 450 nm, 492 nm, 620 nm; Fluorescence: 360 nm, 485 nm), Emission Filter Slide #3 (Fluorescence: 465 nm, 535 nm), Multimode Analysis Software, Power Cable, RS-232 Serial Cable, User’s Manuals FilterMax F5 Multi-Mode Microplate Reader Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. 8 Pages. Click on the microplate icon in the upper left corner and then click FilterMax™ F5 Multi-Mode Microplate Reader: Supplier No. 3 Pages. 3 Pages. It allows the user to choose between monochromator-based optics for flexibility or filter-based optics for sensitivity. Cells were co-transfected with 2 μg total plasmid DNA (1760 ng empty vector or proviral plasmid DNA. The results were calculated using a malondialdehyde (MDA) curve and expressed as nmol MDA/mg protein. Many absorbance assays use a chromogenic substrate, which, upon enzymatic conversion to the final product, results in a compound thatThen 200 µl of 10% (v/v) ammonium hydroxide was added to neutralize the acid and absorbance readings were taken at 405 nm with the FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices. The 0. Instrumentation and Quality Control. Page: 1/4NADPH turnover was measured on FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Francisco, USA) at 340 nm over 30 min at 37 °C. Multi-Mode Detection Platform Overview The FilterMax™ F3 and FilterMax™ F5 Multi-Mode Detection Platforms are computer-controlled multi-mode detectors capable of performing absorbance, luminescence, and fluorescence intensity measurements on microplate samples (Figure 1-1). DMSO was used to dissolve the compounds and was included in the microplate at 0. The microplate drawer door must be held open manually while. I. CatchPoint cGMP Detection Assay Kit. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. Chemiluminescence measurements were performed using a FilterMax TM F5 Multi-Mode Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA). The supernatant was taken and 0. ** Some compatible filter sets are available for purchase from Life. performs within its specifications. This document is provided to customers. 09 CAD. F3/F5 Multi-Mode Microplate Readers. 7. 2 Pages. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Cells were seeded in 24-well plates and cultured. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumAfter incubation for 6 minutes, the mixture was read at 340 nm using a microplate reader (FilterMax F3/F5 Multi-Mode Microplate Readers, Molecular Devices). Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Page 25: FeaturesThe Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seIMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. CloneMatrix. GPCRs. Figure 1. 4 software package (Molecular. 4) onto the FilterMax F5 to be read with SpectraMax® Paradigm® Multi-Mode Detection Platform. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. C1–F5 Filter Set: EX 360/40 nm EM 460/40 nm Optics Position: Gain: Auto Scale to High Wells Scale Well: C1 Scale Value: 30000 Delay after Plate Movement: 100 msec Synergy. Samples (180 μl) were stained with 20 μl of Nile red (2. 5. ScanLater Western Blot Detection System. FilterMax F5 Multi-Mode Microplate Readers, 96 to 384 Well or 6 to 1536 Well, Molecular Devices, LLC (CAT#:. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers can measure absorbance, fluorescence and luminescence. 3. Relative firefly and Renilla luciferase units were measured in a FilterMax F5 Multi-Mode Microplate Reader using the Dual-Luciferase ® Reporter Assay System (Promega) according to the manufacturer’s instructions. 3 Pages. RNA extraction and reverse transcriptase–quantitative PCR. The next day, precultures were diluted to an optical density of 0. FilterMax F5 Multi-mode microplate reader. The multi-mode microplate reader provides flexibility and ease of use for a variety of applications Market Highlights Microplate Readers Market is predicted to project a notable CAGR of 4. MetaMorph NX. com B. SpectraMax Quant dsDNA Assay Kits. FilterMax F3/F5 Multi- Mode Microplate Readers · FilterMax F3 Multi-Mode Microplate Reader Includes Excitation Filter Slide #3 (Absorbance: 405 nm, 450 nm, 492 nm, 620 nm; Fluorescence: of 6 /6. Multi-Mode Reader Comparison Table; Single Mode Readers. 00 USD. 11 mg/mL in PBS, 20 h incubation) using the FilterMax F5 Multi-Mode Microplate Reader, Molecular Devices (Sunnyvale, CA, USA), at 570 nm . A Filter Max F5 Multi-Mode Microplate Reader was used to measure absorbance at 595 nm. The reactions were analyzed in a fluorometer (F5 Multi-Mode Microplate Reader) at 590 nm after a 120-h incubation. Instrument Setup: 1. Important things to consider when purchasing a multimode microplate reader. FilterMax F5 Multi-mode microplate reader. Fluorescence intensity was measured with Filter Max F5 Multi-Mode Microplate Readers (Molecular Devices) at an excitation wavelength of 535 nm and an emission wavelength of 590 nm. The door opens, revealing the transport lock that fastens the microplate drawer to the internal frame of the instrument. Tristar 3 Multimode Microplate Reader. ScanLater Western Blot Detection System. Hit enter to search or ESC to close. Microplate readers, multi-mode, FilterMax™ F3 and F5 Supplier: Molecular Devices Description: The FilterMax™ series of multi-mode microplate readers can be operated as standalone instruments or can be integrated seamlessly with laboratory autom. In addition, the FilterMax F5 Reader is capable of measuring. Easily accessible six-position filter slides enable wavelength selection from 340 to 650nm. After initial incubation, the lysates were incubated with the caspase-1 substrate Ac-YVAD-pNA at 37 °C. The user guide also covers the software features, the maintenance procedures, and the technical specifications of the readers. Yes, the M5 does have a limited slip. FilterMax F3 & F5 Multi-Mode Microplate Readers: SpectraMax i3x Multi-Mode Microplate Reader: SpectraMax Paradigm Multi-Mode Microplate Detection System: Citations: Figures: Catalog Number: Inquire: FilterMax F3 / FilterMax F5: i3x: Inquire: Price: Description: The SpectraMax® i3 Multi-Mode Detection Platform comes with standard. VisionMate 96 (Vial Rack Bottom Label 2D Reader) Ziath. FilterMax F5 Multi-Mode Microplate Reader The FilterMax F5 features absorbance (UV and visible), fluorescence intensity - FI (top and bottom reading), glow luminescence,. * Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Molecular Devices for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above. Based on a rugged, simple design, the VMax Absorbance Microplate Reader offers a mean time between failure (MTBF) of 20,000 hours with 97. The experiment was carried out with four biological replicates and two technical replicates. To do so, perform one of the. com A. MetaFluor Software. Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm), Emission Filter Slide #1 (Fluorescence: 535. 2. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solu. Scan measurement types: endpoint, kinetic, multiple wavelength, linear scan and area scan. Plate Formats:. By category. 3 Pages. ABS Test results. , Sunnyvale, CA, USA) and. 4 Pages. 40266 Email: [email protected]™ F3 and F5 Multimode Microplate Readers, Molecular Devices. 5 fmol/100 μl fluorescein in Fluorescence Intensity-Bottom Read Mode, 15 amol/100 μl Europium in Time-Resolved Fluorescence Mode, 4 mP at 10nM Fluorescein in. 00 USD. 4 Pages. Preconfigured SoftMax® Pro Software protocols (available at softmaxpro. The Beta Plus version of the Sense uniquely combines liquid scintillation counting (LSC) with all common non-radiometric. 40266 Email: drugdiscoverytech@lifetech. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. 425 or. An anti-human HER2/neu human IgG3/κ (Huang and Morrison, 2006) was used as isotype (negative) control. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. 0´ 104 8. Z’-LYTE® Compatible Microplate Reader Documentation Version No. Scan measurement types: endpoint, kinetic, multiple wavelength, linear scan and area scan.